Alamance BIO 111 Genetics of Corn Experiment 1 A Monohybrid CrossBIO 111 – Genetics of Corn Experi

Alamance BIO 111 Genetics of Corn Experiment 1 A Monohybrid Cross

BIO 111 – Genetics of Corn Experiment 1 A Monohybrid Cross

Late Nite Labs

Genetics of Corn

SHORT ANSWER

Experiment 1: A Monohybrid Cross

Directions for BIO 111 – Genetics of Corn Experiment 1 A Monohybrid Cross :

• Use complete sentences and bold the answers and have them in a different color. Failure
  to do so will result in points being deducted.

Lab Results

1. What proportion of purple kernels is expected in the progeny of the monohybrid cross in Experiment 1?
2. Fill in the table below with the data you collected during Experiment 1

• Purple Kernels
• Yellow Kernels

• Row 1
• Row 2
• Row 3
• Row 4
• Row 5

Total

Data Analysis

BIO 111 – Genetics of Corn Experiment 1 A Monohybrid Cross

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3. Use the data you collected in Experiment 1 to calculate the percentage of each phenotype.

4. Suppose you observed 240 progeny that resulted from a monohybrid cross and involved genes that obeyed Mendelian
inheritance, how many heterozygous progeny would you expect to observe?

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Experiment 2: A Dihybrid Cross

Lab Results

Fill in the table below with the data you collected during Experiment 2.

• Purple, Smooth Kernels
• Purple, Wrinkled Kernels
• Yellow, Smooth Kernels
• Yellow, Wrinkled Kernels

• Row 1
• Row 2
• Row 3
• Row 4
• Row 5

Total

Data Analysis

Fill in the following table with the percentage of each type of kernel.

Phenotype

Purple, Smooth
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Proportion of Progeny

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• Purple, Wrinkled
• Yellow, Smooth
• Yellow, Wrinkled

According to your results in Experiment 2, what would be the correct hypothesis to test to see if the traits were inherited through Mendelian genetics? Record this answer for use in a later question BIO 111 – Genetics of Corn Experiment 1 A Monohybrid Cross.

For the cross in Experiment 2, how many degrees of freedom are there? Record this answer for use in a later question.

Determine the ?2 value for Experiment 2 using the following equation. Record your answer for use in a later question.
Phenotype

• Observed
• Expected

(O­E) 2

(O­E)

(O­E) 2/E

• Purple, smooth
• Purple, winkled
• Yellow, smooth
• Yellow, wrinkled

Sum

Use the degrees of freedom and ?2 value from earlier questions to determine the p value for Experiment 2 using the table of critical values for ?2 . Record your answer for use in a later question. BIO 111 – Genetics of Corn Experiment 1 A Monohybrid Cross

Conclusions

1. Use the hypothesis, degrees of freedom, ?2 , and p value determined in earlier questions to conclude whether corn #2 is
following Mendelian genetics. Explain your reasoning.

2. If you crossed Cat #1 and Cat #2 and observed two unlinked traits that follow Mendelian genetics, what must the genotype
and phenotype of Cat #2 be?

Hair length:

• Dominant = long hair (L)
• Recessive = short hair (l)

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Coat color:

• Dominant = white (W)
  Recessive = some color (w)

In a test population of 1000 people, 200 display albinism. You hypothesize that this trait follows Mendelian genetics with a 3:1 ratio of non­albinism to albinism. Use the ?2 test to determine whether the data support the hypothesis or not. Show your steps and explain your reasoning.
?2 = ? [(O – E)2 /E where O is the observed number of individuals in a defined class, and E is the expected number of individuals in a defined class based on the hypothesis. The ? symbol indicates that you should sum the results for each defined class.

BIO 111 – Genetics of Corn Experiment 1 A Monohybrid Cross Sources

Pierce et al., Genetics 2nd Edition.

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BIO 111 – Genetics of Corn Experiment 1 A Monohybrid Cross

Solution: